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KMID : 0545120050150020428
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.428 ~ p.433
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Identification of Bacteriocin-Producing Lactobacillus paraplantarum First Isolated from Kimchi
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Lee JH
Kim MJ/Jeong DW
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Abstract
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A bacteriocin-producing lactic acid bacterium with inhibitory activity against the growth of Lactobacillus plantarum was isolated from kimchi a traditional Korean fermented vegetable. For the identification of the isolate its 16S rDNA was sequenced. As a result the sequence showed 99% homology with those from Lactobacillus paraplantarum Lb. plantarum and Lactobacillus pentosus. For further identification of the isolate the sequence of its 16S/23S rDNA spacer region was determined and the sequence matched perfectly with that of Lb. paraplantarum. SDSPAGE fingerprinting of whole-cell proteins of the isolate was almost identical with that of Lb. paraplantarum. The isolation and identification of Lb. paraplantarum suggest that Lb. paraplantarum is one of the lactic acid bacteria involved in kimchi fermentation.
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